R110 azide, 5- isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| Please inquire about availability of this reagent. | ||||
R110 (rhodamine 110) azide, solid and 10 mM solution in DMSO, labeling reagent for Click chemistry. Pure 5-isomer.
Dye of similar photophysical properties with fluorescein, but more photostable. Can replace Alexa Fluor 488, DyLight 488, FAM (fluorescein).
Recommended protocol
General properties
| Appearance: | red powder / yellow solution |
| Molecular weight: | 456.45 |
| Molecular formula: | C24H20N6O4 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 496 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
Product citations
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
- Shieh, P.; Siegrist, M.S.; Cullen, A.J.; Bertozzi, C.R. Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes. Proceedings of the National Academy of Sciences of the U.S.A., 2014, 111(15), 5456–5461. doi: 10.1073/pnas.1322727111
- Okholm, A.; Kjems, J.; Astakhova, K. Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes. RSC Advances, 2014, 4(86), 45653–45656. doi: 10.1039/c4ra07165d
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