R110 azide, 5- isomer

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R110 (rhodamine 110) azide, solid and 10 mM solution in DMSO, labeling reagent for Click chemistry. Pure 5-isomer.

Dye of similar photophysical properties with fluorescein, but more photostable. Can replace Alexa Fluor 488, DyLight 488, FAM (fluorescein).

5-R110 azide structure
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R110 azide, 6- isomer

Rhodamine 110 (R110) azide for Click chemistry, 6-isomer.

General properties

Appearance: red powder / yellow solution
Molecular weight: 456.45
Molecular formula: C24H20N6O4
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 496
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9

Product citations

  1. Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
  2. Shieh, P.; Siegrist, M.S.; Cullen, A.J.; Bertozzi, C.R. Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes. Proceedings of the National Academy of Sciences of the U.S.A., 2014, 111(15), 5456–5461. doi: 10.1073/pnas.1322727111
  3. Okholm, A.; Kjems, J.; Astakhova, K. Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes. RSC Advances, 2014, 4(86), 45653–45656. doi: 10.1039/c4ra07165d
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