AF 430 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A0830 | 1 mg | $125 | in stock | |
| B0830 | 5 mg | $325 | in stock | |
| D0830 | 25 mg |
$950
|
in stock | |
| E0830 | 50 mg |
$1625
|
in stock | |
| F0830 | 100 mg |
$2090
|
in stock |
AF 430 is a fluorescent dye. It can be excited by 405 nm violet laser or 445 nm laser paired, for example, with 510/80 nm bandpass filter.
Azide moiety allows quick and effective labeling and detection of terminal alkynes via a copper-catalyzed click reaction (CuAAC) or of strained cyclooctynes via a copper-free click chemistry reaction (SPAAC). Mild reaction conditions are suitable for most biomolecules, cells, and tissues. The product is water soluble and insensitive to pH changes between pH 4 and pH 10.
Absorption and emission spectra of AF 430
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AF 488 alkyne
Alkyne derivative of AF 488 dye for copper-catalyzed cycloaddition to azides. Pure 5-isomer.Copper(II)-THPTA catalytic buffer, 1.5x
Ready-to-use catalytic buffer containing сopper(II) and water-soluble THPTA ligand. It is suitable for click chemistry modification of proteins.AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.General properties
| Appearance: | yellow solid |
| Molecular weight: | 623.69 |
| Molecular formula: | C25H29N5F3KO6S |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
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