Lumiprobe offers a selection of kits for the quantification of biomolecules: nucleic acids, and proteins. The measurement is based on the selective binding of dyes with the target molecules, and corresponding fluorescent response.
The measurement results correlate well with other measurement methods. The measurements are reproducible. The method has a wide dynamic range up to three orders of magnitude.
There are kits specifically designed for common fluorometers like QuBit®, but any fluorescent reader with green (fluorescein or FAM, 490/520 nm) channel can be used for the measurements with proper calibration - all calculations are done intuitively using our DNA/Protein quantification calculator tool.
The core of this Protein Quantification Kit is a very sensitive ProteOrange fluorescent dye. This dye is suitable for use with spectrofluorometers and microplate readers. The kit is optimised for protein quantification, with detection level as low as 10 ng/mL of protein in final solution.
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Ready kit for accurate DNA quantification with Qubit® fluorometer in the broad range from 100 pg/µL to 1000 ng/µL. Common contaminants are well tolerated in the assay, only 1-20 µL of experimental sample is required. Measurements are performed at room temperature.