Lumiprobe, LLC. Dyes & more for molecular biology
Quick navigation
| select technology: |
| or |
| select product group: |
More information
| Product Catalog |
| Alphabetical List of Products |
| Recommended Protocols |
| Shipping & Returns |
| International distributors |
| Privacy Notice |
| Terms of Use |
| Contact Us |
My account
| Shopping cart: no items |
SYBR Green I Gel Staining Solution, 10000x
| Lot No | Quantity | Price | |
|---|---|---|---|
| 20010 | 1 mL | $ 180.00 | |
| 20010-2 | 2 x 1 mL | $ 195.00 | |
| 30010-3 | 3 x 1.5 mL | $ 415.00 | |
| 30010-5 | 5 x 1.5 mL | $ 675.00 |
SYBR Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels.
A comparison between Ethidium Bromide and SYBR Green I
| Feature | Ethidium Bromide | SYBR Green I |
|---|---|---|
| Fluorescence | Red (615 nm) | Green (524 nm) |
| Excitation maximum | 302 nm | 454 nm |
| Excitation light source | UV only | Blue light or UV |
| Sensitivity | 2 ng / band(dsDNA) 100 ng / band (RNA) | 0.08 ng / band (dsDNA) 1-2 ng / band (oligonucleotides) |
| Health hazard | High | Low |
There are three basic protocols for DNA staining in gels: gel soaking, gel prestaining, and sample prestaining.
Protocols available: View protocol | Download as pdf
Spectral properties
| excitation maximum, λmaxex, nm: | 454 |
| extinction at λmaxex | 73,000 (TE, pH 7.5) |
| fluorescence maximum, λmaxfluor, nm: | 524 |
| fluorescence quantum yield: | 0.8 |
[1]Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
Order now. Receive within 3-8 days in any place around the world.
Pay with
Featured
Dye equivalence charts
Replace your fluorescent dyes with effecient & affordable probes from Lumiprobe