sulfo-Cyanine7 NHS ester
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
15320 | 1 mg | $125.00 | in stock | |
25320 | 5 mg | $325.00 | in stock | |
45320 | 25 mg | $850.00 | in stock | |
55320 | 50 mg | $1490.00 | in stock | |
65320 | 100 mg | $1990.00 | in stock |
Water-soluble near-infrared dye sulfo-Cyanine7, an amine-reactive succinimide ester.
sulfo-Cyanine7 is an improved analog of Cy7® fluorophore with quantum yield improved by 20%, and higher photostability. This fluorescent dye is especially useful for NIR imaging.
Near-infrared fluorescent imaging takes advantage of the transparency of biological tissues at a particular range of wavelengths. The method is non-destructive and allows to monitor of the distribution of various labeled molecules in live organisms.
sulfo-Cyanine7 NHS ester reagent allows to prepare sulfo-Cyanine7-labeled biomolecules, such as proteins, with ease. Dye-labeled molecules can be subsequently used for various research and drug design-related experiments.
This reagent has high water solubility and is especially useful for labeling delicate proteins and proteins prone to denaturation. Non-sulfonated Cyanine7 NHS ester soluble in the organic phase is also available.
sulfo-Cyanine7 absorbance and emission spectra
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General properties
Appearance: | dark green powder |
Molecular weight: | 844.05 |
CAS number: | 1603861-95-5 (potassium salt); 1604244-45-2 (inner salt); 477908-53-5 (N-Ethyl) |
Molecular formula: | C41H46N3KO10S2 |
Solubility: | good in water, DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 750 |
ε, L⋅mol−1⋅cm−1: | 240600 |
Emission maximum, nm: | 773 |
Fluorescence quantum yield: | 0.24 |
CF260: | 0.04 |
CF280: | 0.04 |
Product citations
- Feng, X.A.; Ness, K.M.; Liu, C.; Ahmed, I.; Bowman, G.D.; Ha, T.; Wu, C. GAGA Factor Overcomes 1D Diffusion Barrier by 3D Diffusion in Search of Nucleosomal Targets. bioRxiv, 2023, preprint. doi: 10.1101/2023.07.14.549009
- Sandberg, E.; Piguet, J.; Kostiv, U.; Baryshnikov, G.; Liu, H.; Widengren, J. Photoisomerization of Heptamethine Cyanine Dyes Results in Red-Emissive Species: Implications for Near-IR, Single-Molecule, and Super-Resolution Fluorescence Spectroscopy and Imaging. J Phys Chem B, 2023, 127(14), 3208–3222. doi: 10.1021/acs.jpcb.2c08016
- Birnbaum, L.A.; Sullivan, E.C.; Do, P.; Uricoli, B.; Raikar, S.S.; Porter, C.C.; Henry, C.J.; Dreaden, E.C. Multicolor Light-Induced Immune Activation via Polymer Photocaged Cytokines. Biomacromolecules, 2023, 24(3), 1164-1172. doi: 10.1021/acs.biomac.2c01207
- Hao, L.; Zhao, R.T.; Welch, N.L.; Tan, E.K.W.; Zhong, Q.; Harzallah, N.S.; Ngambenjawong, C.; Ko, H.; Fleming, H.E.; Sabeti, P.C.; Bhatia, S.N. CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics. Nature Nanotechnology, 2023, 18, 798-807. doi: 10.1038/s41565-023-01372-9