FAM amine, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 141C0 | 1 mg | $110 | in stock | |
| 241C0 | 5 mg | $210 | in stock | |
| 441C0 | 25 mg |
$410
|
in stock | |
| 541C0 | 50 mg |
$695
|
in stock | |
| 641C0 | 100 mg |
$1190
|
in stock |
Fluorescein (FAM) amine, isomerically pure dye (5-isomer). Unlike fluoresceinamine, this reagent contains a linker arm with an aliphatic amine group that is more reactive. It can react with electrophilic reagents like activated esters or epoxides, and be involved in enzymatic transamination.
FAM absorbance and emission spectra
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AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.EDC (N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide), coupling grade
Water soluble carbodiimide for the coupling of carboxylic acids with amines.General properties
| Appearance: | yellow solid |
| Molecular weight: | 510.97 |
| CAS number: | 2183440-41-5 (hydrochloride), 138589-19-2 |
| Molecular formula: | C27H27N2ClO6 |
| IUPAC name: | 6-aminohexylaminocarbonylfluorescein hydrochloride |
| Solubility: | good in methanol, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
Product citations
- Sotnikov, D.V.; Byzova, N.A.; Zherdev, A.V.; Dzantiev, B.B. Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein. International Journal of Molecular Sciences, 2023, 24(23), 16967. doi: 10.3390/ijms242316967
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