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Alkyne Amidite, 5'-terminal
| Lot No | Quantity | Price | |
|---|---|---|---|
| 22260 | 250 mg | $ 230.00 | |
| 42260 | 1 g | $ 450.00 | |
| 62260 | 5 g | $ 1400.00 |
Amidite for the synthesis of alkyne-modified oligonucleotides containing 5'-terminal modification. Oligonucleotides can be used for Click Chemistry modification (see or download protocol).
| General properties | |
|---|---|
| Appearance: | white crystalline solid |
| Molar weight: | 409.50 |
| Oligonucleotide synthesis conditions | |
| Synthesizer compatibility | all synthesizers using phosphoramidite chemistry |
| Coupling time | 5 min recommended |
| Deblock conditions | Standard (6h in aq. ammonia at 60°C) |
| Oligo purification | PAAG electrophoresis or HPLC |
| Oligo storage | not different from standart oligonucleotides |
| Quality control | |
| NMR (1H, 31P): | 95+% |
| Functional testing (oligo synthesis + oligo modification with CuAAC): | pass |
| Recommended protocols | |
| Click-chemistry labeling of oligonucleotides and DNA | HTML | PDF |
| Storage conditions | |
| Store at -20°C. Desiccate. | |
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