3Dye™ 2D DIGE Kits

Because of IP issues, reagents for 2D differential electrophoresis are unavailable in US&Canada until April 20, 2015.

Lumiprobe 3Dye™ 2D DIGE Kits are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allows for high efficient detection and high resolution of minor proteins on 2D gel electrophoresis.

2D DIGE Gel containing Cy2, Cy3, and Cy5 labeled proteins

The kits are compatible to all imagers capable of detection of Cy2, Cy3, and Cy5 dyes. The gels labeled with Lumiprobe 2D DIGE Kits are ready for subsequent mass-spectrometrical analysis.

Each 3Dye™ 2D DIGE kit contains reactive NHS esters of Cyanine2, Cyanine3, and Cyanine5 dyes, and labeling grade dimethylformamide (DMF) solvent. Cyanine2 can be used as a reference which can be added to both samples.

Reactive dyes are contained in lyophilized form, which prolonges shelf-life of the kits. Each tube contains exactly the same quantity of reactive dyes (variation within 10%). Re-constitution of the NHS esters with included DMF guarantees that labeling efficiency of Cyanine2, Cyanine3, and Cyanine5 remains the same during entire term of kit use.

Please order kit components separately:

Cy2 fluor (5 nmol, 10 nmol, or 25 nmol)
Cy3 fluor (5 nmol, 10 nmol, or 25 nmol)
Cy5 fluor (5 nmol, 10 nmol, or 25 nmol)
DMF labeling grade solvent (1 mL)

Recommended protocol

Recommended protocol is available. However, if you are using other labeling dyes for differential proteomics, you can switch seamlessly, and continue using your standard protocols.

Performance evaluation

Two lysates of different Helicobacter Pylori strains were analyzed using dyes produced by competitor (left panel) and Lumiprobe 3Dye™ 2D DIGE Kit (right panel). The images taken by GE Healthcare Typhoon 9410 imager are shown below.

A comparison between competitor and Lumiprobe 3Dye™ 2D DIGE kits

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Cy is a trademark of GE Healthcare.

General properties

Appearance: Dyes: small amounts of material in plastic vials. DMF: colorless liquid.
Quality control: Functional testing (protein labeling) with quantity measurement
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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