Click Chemistry reagents

Lumiprobe provides a comprehensive list of Click Chemistry reagents - azides, alkynes, and auxiliary components.

(are you new to Click Chemistry? Learn more about this technology!)

Fluorescent dye azides

These azides can be used as reagents for Click Chemistry labeling of alkyne-containing molecules.

• Cy3 azide, 10 mM in DMSO - the lowest-wavelength cyanine dye
• Cy3.5 azide, 10 mM in DMSO - cyanine dye azide, spectrum is intermediate between Cy3 and Cy5
• Cy5 azide, 10 mM in DMSO - one of the most standard cyanine fluorophores
• Cy5.5 azide, 10 mM in DMSO - near IR dye azide
• 5-FAM azide, 10 mM in DMSO - one of most common fluorophores, single isomer.
• 6-FAM azide, 10 mM in DMSO - another isomer of FAM
• 5-JOE azide, 10 mM in DMSO - fluorescein derivative, analog of TET
• 5-R110 azide, 10 mM in DMSO - more photostable analog of FAM
• 6-R110 azide, 10 mM in DMSO - another isomer of R110
• 6-ROX azide, 10 mM in DMSO - rhodamine X azide
• 5-TAMRA azide, 10 mM in DMSO - tetramethylrhodamine azide

Activated ester reagents

Activated ester reagents can be used to convert amino groups, present in many biomolecules, into azides and alkynes, which are reactive in Click Chemistry

• Azidobutyric acid NHS ester - a reagent converting amino groups to Click-ready azides
• Pentynoic acid STP ester - a reagent converting amino groups to alkynes ready to Click Chemistry

Alkyne amidites (phosphoramidites) for solid phase DNA/RNA synthesis

Since azido group is incompatible with oligo synthesis, only amidites containing alkynes can be used to prepare oligonucleotides for Click Chemistry.

• Alkyne amidite, 5'-terminal - a convenient labeling reagent for 5'-alkyne labeling
• Alkyne amidite, hydroxyprolinol - alkyne phosphoramidite containing DMT group, for cartrige purification of alkyne-modified oligos.

Auxiliary Click Chemistry reagents

Click Chemistry requires Copper(I)-catalysis to take place. To prepare the catalyst, Lumiprobe recommends mixing Cu(II)-TBTA complex with ascorbic acid prior to reaction. Please see our recommended protocols.

• Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO - catalyst, stable Cu(II)-form
• Ascorbic acid - recommended reagent to activate stable Cu(II) form of the catalyst prior to reaction